Superior confocal Raman imaging system

alpha300 R from WITec

The imaging capabilities of the alpha300 R fulfill the highest requirements of confocal Raman imaging with superior performance in speed, sensitivity and resolution. These unique characteristics have established the alpha300 R the preeminent confocal Raman imaging system on the market.

Raman spectral imaging
Image stacks
3D imaging and depth profiling
Highly linear piezo-driven scan-stage with a scan range of 200 x 200 x 20 μm
Exceptional accuracy over the entire travel and scan range (0.03 % linearity)

While long recognized as the state-of-the-art imaging system, ongoing development resulting from WITec's innovative spirit has kept the alpha300 R at the forefront of Raman microscopy and set the benchmark in terms of imaging capability as well as spectral quality, spatial resolution, ease-of-use and compatibility with other measurement techniques.

The flexibility of the alpha300 R series allows the system adapt to all requirements, combine different imaging techniques and to evolve to meet new or expanded needs.

The alpha300 R offers the following extensions:

alpha300 R+ for automated measurements:

  • Automated motorized sample positioner in x-, y-, and z-direction, 25 mm travel range (50 mm travel range optional)
  • Automated confocal Raman Imaging (25 x 25 mm; optional 50 x 50 mm)
  • Automated multi-area and multi-point measurements
  • 2D and 3D Raman mapping
  • Raman depth profiling
  • Autofocus

alpha500 R for automated large sample investigations:

  • Motorized scan stage for large-area confocal Raman mapping (150 x 100 mm) with automated sample positioner in x-, y-, and z-direction
  • Motorized z-stage system for automated approach
  • Automated large area confocal Raman Imaging (150 x 100 mm)
  • Automated multi-area and multi-point measurements
  • 2D and 3D Raman mapping
  • Raman depth profiling
  • Autofocus

Operation Modes:

  • Raman spectral imaging: acquisition of a complete Raman spectra at every image pixel
  • Planar (x-y-direction) and depth scans (z-direction)
  • Image stacks: 3D confocal Raman Imaging
  • Time series
  • Single point Raman spectrum acquisition
  • Single-point depth profiling
  • Ultrafast Raman Imaging (1300 spectra per second) optional available
  • Confocal fluorescence microscopy
  • Bright Field Microscopy
  • Dark Field, Phase Contrast and DIC optional
  • Upgradable for epi-fluorescence applications

Microscope Features:

  • Research grade optical microscope with 6x objective turret
  • Video system: eyepiece color video camera
  • LED white-light source for Köhler illumination
  • Manual sample positioning in x- and y-direction
  • Piezo-driven scan stage (scan range 200 x 200 x 20 ?m) (other ranges available)
  • Ultra High Throughput Spectrometer UHTS300
  • f/4 300 mm focal length imaging spectrometer specifically designed for Raman microscopy and applications with low light intensities
  • Lens-based, excitation optimized spectrometer (Vis or NIR)
  • Ultrahigh-throughput optical system
  • Superior peak shape conservation

Sample Size:

  • Usually 120 mm in x- and y-direction, 25 mm in height; adapter for larger heights available

Computer Interface:

  • WITec software for instrument and measurement control, data evaluation and processing
Life Science

From measurements in liquids to solid samples or soft tissues in life science varied samples are analyzed on a regular basis. With their convenient handling and versatile analytical capabilities the flexible WITec imaging systems provide the opportunity to adjust the imaging technique to changing requirements and are particularly well-suited for life science.

Pharmaceutics, cosmetics

The development and production of drug delivery systems requires efficient and reliable control mechanisms to ensure the quality of the final products. These products can vary widely in composition and application. Therefore analytical tools such as the WITec imaging systems that provide both comprehensive chemical characterization and the flexibility to adjust the method to the investigated specimen are preferred in pharmaceutical research.

Materials science

Materials science is a diverse field including the development and testing of new substances, as well as the refinement of manufacturing processes and quality control for existing products. WITec imaging systems are particularly well-suited for comprehensive sample analyses in materials science and provide the opportunity to acquire a thorough knowledge of the sample surface morphology and chemical composition.


WITec confocal Raman imaging systems are excellent analytical tools for the comprehensive investigation of geological samples, such as the identification and characterization of minerals, or in the observation of mineral phase transitions in high and ultra-high pressure/temperature experiments.


WITec imaging systems enable comprehensive sample analysis that provides a thorough characterization of the physical and chemical properties of the polymers on the nanometer scale.

Nano-Carbon and graphene

Nano-carbon materials such as graphene or carbon nano-tubes show immense promise in many applications such as transistors, sensors, and optoelectronics. Flexible and adaptive analytical methods can support effective investigation and accelerate progress in nano-carbon & graphene research and development.

"From the initial enquiry, to purchase and installation, the staff at LOT-QuantumDesign were excellent throughout. They gave an excellent demonstration of the alpha 300R+, which helped us to identify the most appropriate components for the research we intended to perform."

- Dr James Bowen at Birmingham University, School of Chemical Engineering

Dr James Bowen has a WITec Confocal Raman Microscope alpha 300R+ system with large area scanning of up to 25mm x 25mm XY. This is available for any collaboration with other research institutions in the UK or for service contract work within industry. Please visit Dr Bowen's website for further information or call Dr James Bowen on +44 121 414 5080, Email The alpha300R+ purchase was funded by ERDF.

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